# Cryo-EM
**URL:** https://forum.scilifelab.se/c/technologies/cryo-em/32.md
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## [About the Cryo-EM category](https://forum.scilifelab.se/t/about-the-cryo-em-category/86)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\
**Replies:** 0
This topic is a place for Cryo-EM facility users to ask/answer questions and start discussions in a continuous community forum outside of our bi-weekly group meeting and drop-in sessions. We hope that you can use this f…
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## [Some timing tests of the Gatan K3](https://forum.scilifelab.se/t/some-timing-tests-of-the-gatan-k3/139)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\
**Replies:** 5\
**Last updated:** [12 June 2024 06:38 UTC](https://forum.scilifelab.se/t/some-timing-tests-of-the-gatan-k3/139 "2024-06-12T06:38:53Z")
Introduction So while we were testing our new Krios Beta, we found ourselves a little perplexed on what the expected throughput would be with the K3 within EPU. Krios Beta is a G3i with AFIS as well as the Fringe Free I…
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## [Molecular weight information derived from cryo-em micrographs?](https://forum.scilifelab.se/t/molecular-weight-information-derived-from-cryo-em-micrographs/291)
**Author:** [@kohyou92](https://forum.scilifelab.se/u/kohyou92)\
**Replies:** 2\
**Last updated:** [11 June 2024 11:06 UTC](https://forum.scilifelab.se/t/molecular-weight-information-derived-from-cryo-em-micrographs/291 "2024-06-11T11:06:11Z")
Nowadays there are mass photometry techniques that use TIRF-like microscopy technique to measure molecular weight of particles. I wonder if we can derive/deduce molecular weight information directly from cryo-em microgra…
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## [Creating Optics Groups from EPU AFIS data and more](https://forum.scilifelab.se/t/creating-optics-groups-from-epu-afis-data-and-more/122)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\
**Replies:** 10\
**Last updated:** [10 June 2024 05:48 UTC](https://forum.scilifelab.se/t/creating-optics-groups-from-epu-afis-data-and-more/122 "2024-06-10T05:48:19Z")
So you recently used “Faster Acquisition” in EPU for your session, and you also want to try the newest features in RELION which allow you to correct for higher-order aberrations and anisotropic magnification. However, yo…
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## [On the benefits of patch-tracking](https://forum.scilifelab.se/t/on-the-benefits-of-patch-tracking/145)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\
**Replies:** 1\
**Last updated:** [28 February 2021 16:19 UTC](https://forum.scilifelab.se/t/on-the-benefits-of-patch-tracking/145 "2021-02-28T16:19:03Z")
Introduction So yesterday I responded to this tweet: and I am working with some data that needs patch-tracking, so I thought it might be beneficial to elaborate more on my experience comparing coarse whole-image align…
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## [Thinking about CTF](https://forum.scilifelab.se/t/thinking-about-ctf/114)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\
**Replies:** 8\
**Last updated:** [28 August 2020 13:43 UTC](https://forum.scilifelab.se/t/thinking-about-ctf/114 "2020-08-28T13:43:48Z")
One of the question we will ask when setting up collections is what range of defocuses to target for imaging. This is an important question and the answer affects the entire rest of data processing, so careful considera…
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## [A Collection of Cryo-EM Course Resources](https://forum.scilifelab.se/t/a-collection-of-cryo-em-course-resources/87)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\
**Replies:** 3\
**Last updated:** [12 June 2020 10:08 UTC](https://forum.scilifelab.se/t/a-collection-of-cryo-em-course-resources/87 "2020-06-12T10:08:06Z")
Here is a collection of links to various course materials given over the years at various institutes. I will do my best to keep this up-to-date. I hope you find it useful! EMBO Cryo-EM Heidelberg Folder 2010 2012 2014 …