# Latest **URL:** https://forum.scilifelab.se/latest.md [Latest](https://forum.scilifelab.se/latest.md) · [Categories](https://forum.scilifelab.se/categories.md) · [Tags](https://forum.scilifelab.se/tags.md) --- ## [Overview of SciLifeLab COVID-19 communication channels](https://forum.scilifelab.se/t/overview-of-scilifelab-covid-19-communication-channels/24)
**Author:** [@ewels](https://forum.scilifelab.se/u/ewels)\ **Replies:** 2\ **Last updated:** [18 March 2024 13:24 UTC](https://forum.scilifelab.se/t/overview-of-scilifelab-covid-19-communication-channels/24 "2024-03-18T13:24:02Z")
The SciLifeLab Data Centre has set up communication tools for the Covid-19 work. The key channels are as follows: SciLifeLab Slack channels Slack is good for fast-paced discussions organized in different channels. The S… --- ## [How can Single Cell RNA Sequence Data Analysis be improved?](https://forum.scilifelab.se/t/how-can-single-cell-rna-sequence-data-analysis-be-improved/373)
**Author:** [@halcyoncv](https://forum.scilifelab.se/u/halcyoncv)\ **Replies:** 0\ **Last updated:** [10 September 2024 06:20 UTC](https://forum.scilifelab.se/t/how-can-single-cell-rna-sequence-data-analysis-be-improved/373 "2024-09-10T06:20:20Z")
Hello Community Member, I am working on a single cell RNA sequencing project & am looking for advice on optimizing my data analysis pipeline. I have already performed the initial quality control steps and basic cluster… --- ## [Looking for Advice for Sophisticated CRISPR-Cas9 Uses in Gene Therapy Research](https://forum.scilifelab.se/t/looking-for-advice-for-sophisticated-crispr-cas9-uses-in-gene-therapy-research/365)
**Author:** [@Elina](https://forum.scilifelab.se/u/Elina)\ **Replies:** 0\ **Last updated:** [24 July 2024 08:01 UTC](https://forum.scilifelab.se/t/looking-for-advice-for-sophisticated-crispr-cas9-uses-in-gene-therapy-research/365 "2024-07-24T08:01:31Z")
Hello Everyone :hugs:, I’m a molecular biology PhD candidate now, specialising on genetic engineering and gene therapy. I have been actively investigating the therapeutic applications of CRISPR-Cas9 technology over the … --- ## [Why I am unable to see my post?](https://forum.scilifelab.se/t/why-i-am-unable-to-see-my-post/343)
**Author:** [@Lawatson](https://forum.scilifelab.se/u/Lawatson)\ **Replies:** 0\ **Last updated:** [13 June 2024 16:57 UTC](https://forum.scilifelab.se/t/why-i-am-unable-to-see-my-post/343 "2024-06-13T16:57:19Z")
Hello there, Recently I posted a query on this thread- http://forum.scilifelab.se/t/exploring-cooperation-and-understanding-for-plant-genomics-applications-of-crispr-cas9/341/1 After few minutes, I got a notificatio… --- ## [Brooke Jessica Kaio](https://forum.scilifelab.se/t/brooke-jessica-kaio/190)
**Author:** [@brookejessicakaio](https://forum.scilifelab.se/u/brookejessicakaio)\ **Replies:** 2\ **Last updated:** [13 June 2024 12:27 UTC](https://forum.scilifelab.se/t/brooke-jessica-kaio/190 "2024-06-13T12:27:45Z")
Brooke Jessica Kaio - In my opinion, SciLifeLab is a national resource of unique technologies --- ## [Some timing tests of the Gatan K3](https://forum.scilifelab.se/t/some-timing-tests-of-the-gatan-k3/139)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\ **Replies:** 5\ **Last updated:** [12 June 2024 06:38 UTC](https://forum.scilifelab.se/t/some-timing-tests-of-the-gatan-k3/139 "2024-06-12T06:38:53Z")
Introduction So while we were testing our new Krios Beta, we found ourselves a little perplexed on what the expected throughput would be with the K3 within EPU. Krios Beta is a G3i with AFIS as well as the Fringe Free I… --- ## [Molecular weight information derived from cryo-em micrographs?](https://forum.scilifelab.se/t/molecular-weight-information-derived-from-cryo-em-micrographs/291)
**Author:** [@kohyou92](https://forum.scilifelab.se/u/kohyou92)\ **Replies:** 2\ **Last updated:** [11 June 2024 11:06 UTC](https://forum.scilifelab.se/t/molecular-weight-information-derived-from-cryo-em-micrographs/291 "2024-06-11T11:06:11Z")
Nowadays there are mass photometry techniques that use TIRF-like microscopy technique to measure molecular weight of particles. I wonder if we can derive/deduce molecular weight information directly from cryo-em microgra… --- ## [Creating Optics Groups from EPU AFIS data and more](https://forum.scilifelab.se/t/creating-optics-groups-from-epu-afis-data-and-more/122)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\ **Replies:** 10\ **Last updated:** [10 June 2024 05:48 UTC](https://forum.scilifelab.se/t/creating-optics-groups-from-epu-afis-data-and-more/122 "2024-06-10T05:48:19Z")
So you recently used “Faster Acquisition” in EPU for your session, and you also want to try the newest features in RELION which allow you to correct for higher-order aberrations and anisotropic magnification. However, yo… --- ## [BEAS-2B cell culture and MTT assay variability](https://forum.scilifelab.se/t/beas-2b-cell-culture-and-mtt-assay-variability/258)
**Author:** [@anacouto](https://forum.scilifelab.se/u/anacouto)\ **Replies:** 0\ **Last updated:** [12 December 2022 19:15 UTC](https://forum.scilifelab.se/t/beas-2b-cell-culture-and-mtt-assay-variability/258 "2022-12-12T19:15:13Z")
Good evening, everyone. Does anyone have experience with culturing BEAS-2B cells (broncho-epithelial cell lines)? I’m trying to do a MTT assay since September with cisplatin at stock concentration 3.33mM (serial diluti… --- ## [On the benefits of patch-tracking](https://forum.scilifelab.se/t/on-the-benefits-of-patch-tracking/145)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\ **Replies:** 1\ **Last updated:** [28 February 2021 16:19 UTC](https://forum.scilifelab.se/t/on-the-benefits-of-patch-tracking/145 "2021-02-28T16:19:03Z")
Introduction So yesterday I responded to this tweet: and I am working with some data that needs patch-tracking, so I thought it might be beneficial to elaborate more on my experience comparing coarse whole-image align… --- ## [Thinking about CTF](https://forum.scilifelab.se/t/thinking-about-ctf/114)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\ **Replies:** 8\ **Last updated:** [28 August 2020 13:43 UTC](https://forum.scilifelab.se/t/thinking-about-ctf/114 "2020-08-28T13:43:48Z")
One of the question we will ask when setting up collections is what range of defocuses to target for imaging. This is an important question and the answer affects the entire rest of data processing, so careful considera… --- ## [A Collection of Cryo-EM Course Resources](https://forum.scilifelab.se/t/a-collection-of-cryo-em-course-resources/87)
**Author:** [@dustin.morado](https://forum.scilifelab.se/u/dustin.morado)\ **Replies:** 3\ **Last updated:** [12 June 2020 10:08 UTC](https://forum.scilifelab.se/t/a-collection-of-cryo-em-course-resources/87 "2020-06-12T10:08:06Z")
Here is a collection of links to various course materials given over the years at various institutes. I will do my best to keep this up-to-date. I hope you find it useful! EMBO Cryo-EM Heidelberg Folder 2010 2012 2014 … --- ## [Welcome to the SciLifeLab Forum](https://forum.scilifelab.se/t/welcome-to-the-scilifelab-forum/34)
**Author:** [@ewels](https://forum.scilifelab.se/u/ewels)\ **Replies:** 0\ **Last updated:** [24 March 2020 17:14 UTC](https://forum.scilifelab.se/t/welcome-to-the-scilifelab-forum/34 "2020-03-24T17:14:10Z")
This is the SciLifeLab web forum for discussions related to the SciLifeLab infrastructure and related research. This provides SciLifeLab personnel with a forum to publish public information and discussion. Pages can be … --- ## [SciLifeLab Forum User Guide](https://forum.scilifelab.se/t/scilifelab-forum-user-guide/33)
**Author:** [@ewels](https://forum.scilifelab.se/u/ewels)\ **Replies:** 0\ **Last updated:** [24 March 2020 16:49 UTC](https://forum.scilifelab.se/t/scilifelab-forum-user-guide/33 "2020-03-24T16:49:59Z")
Welcome! As a new user of the SciLifeLab Forum, we hope you will find this site intuitive and clearly structured, but here is some guidance to get you started: Basic Terms Used by Discourse Browsing the Forum Topic li…