Hello Everyone 
,
I’m a molecular biology PhD candidate now, specialising on genetic engineering and gene therapy. I have been actively investigating the therapeutic applications of CRISPR-Cas9 technology over the past few years. Even though I’ve learnt a lot about theory and have some real-world experience, I’m looking for additional in-depth advice and direction from professionals in this sector.
I’m particularly curious about the following CRISPR-Cas9 application aspects:
Mitigate Off-Target Effects: Even with the progress made in CRISPR technology, mutations that are off-target continue to pose a serious problem. What are the most recent tactics or resources being employed to lessen these effects? 
Do you have any recommendations for recent studies or publications?
Transport Mechanisms: Successful gene editing depends on the effective transport of CRISPR components to target cells. Which delivery techniques are currently being researched or used in clinical settings that show the greatest promise? How do the effectiveness and safety of different approaches compare?
Ethical Considerations: As genetic engineering advances, it becomes more crucial to take ethical issues into account. What steps are academics and organisations taking to resolve the moral conundrums raised by gene editing, especially when it comes to human subjects? Are here any established rules or models that you think might be especially helpful?
Opportunities for Collaboration: I would also like to investigate the possibility of collaborating with other scholars or organisations that are engaged in related topics. Let me know if anyone is willing to talk about potential for collaborative research or resource sharing.
Any knowledge, firsthand knowledge, or resources you could provide on these subjects would be highly appreciated. Your knowledge and direction will be very helpful to me as I proceed to work through this fascinating yet uipath challenging topic.
Thank you in advance.