Hi Dustin,
This is a really cool tool! I have been playing around a bit now and have some questions on exactly how to read the output graphs - hope you can help me
First, I entered some parameters which are very much like a recent case where we collected at KI.
(top panel in attached pdf)
Though the actual data has not been processed at the physical size, in the playing around using your tool I just imagined to have the particles in a box, not downsampled.
The range of defocus values I just put as the nominal values from collection, not the actual from micrographs after CTF estimation.
(the second and third panels in the attached pdf)
But it seems like some frequencies are actually not covered by this range of defocus values?
Next, I am not exactly sure how to read the graphs related to box size and aliasing.
In the fourth graph panel in the pdf, should I read that the aliasing is only the invariant/vertical part of the curves for the respective defocus values?
If so, in my case here it is only the particles of defocus values around 0.4 um I can expect some aliasing?
And the fifth graph panel, the lower one, should I read this as my box of 800 pix is actually a bit too small to avoid aliasing in particles with 1.5 um defocus or higher?
Finally, the last graph on delocalisation, to me looks like the current box size of 800px (say for a particle of 400px) is too small to encompass the delocalized signal in the higher defocus particle images?
(bottom panel in pdf).
Thanks a lot for your help.
Btw, I was not able to upload more than one picture in my post, hence the single pdf file. Can this maybe be changed for users so that we can add pictures similar to your original post here on CTF?
Best,
Rasmus