Creating Optics Groups from EPU AFIS data and more

dustin.morado · 29 June 2020 14:39

So it was brought to my attention from the cryoSPARC documentation that template positions are indicated in the filenames of EPU movies. However, upon further inspection EPU is not creating different tags for different holes (hopefully this is something they will be adding soon):

github.com/DustinMorado/EPU_group_AFIS

EPU can indicate image shift group in file names

opened 11:11AM - 29 Jun 20 UTC

closed 02:31PM - 29 Jun 20 UTC

Guillawme

Hi, This is very helpful, thank you! I especially like the visualization of i…mage shift groups. But it seems a bit crazy that one has to do all this to figure out which movies belong to which image shift group. Surely, EPU must be able to indicate the image shift group in filenames? It would then be easy to match files belonging to only one group during import in RELION, to import each group independently. So, I tried to find whether the image shift group information is already present in the file name, and according to [CryoSPARC's documentation](https://cryosparc.com/docs/tutorials/ctf-refinement#exposure-groups) this is indeed the case: the first number after `Data` in the file name supposedly encodes image shift group. I checked in a bunch of movies I have that were recorded with EPU, and this first number after `Data` indeed only ever takes 4 different values across all my movies, so this is consistent with an image shift group (although not obvious at all, since these numbers look completely arbitrary and are big). I am not sure this is done by default, maybe it was set this way in the EPU session that produced this particular set of movies I have? But I would suggest you check what your EPU does, because it seems you can maybe make your life a lot easier to assign image shift groups.

Here’s the link to the cryoSPARC information in case you don’t have the patience to read through the issue

But regardless, this was news to me and so I wanted to share the information to you all as well in case you find it useful.

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Creating Optics Groups from EPU AFIS data and more

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